Bean Spread Enriched with Spelt as a Novel Source of Bioactive Peptides with Potential Anti-Metabolic Syndrome Properties

Bean spreads enriched with 10, 20, or 30% of spelt grain were analyzed in the study as a novel product high nutraceutical potential. The hydrolyzed vitro gastrointestinal conditions. highest peptide content was noted after last step hydrolysis spread 10% (1.64 mg/mL). fraction molecular mass < 3.0 kDa obtained from this hydrolyzate also characterized by (1.50 mg/mL) and antioxidant properties. value IC50 against ABTS•+ 0.078 mg/mL. Fe2+ chelating activity 0.056 Moreover, trials show inhibitory enzymes involved development metabolic syndrome (with values 0.072 mg/mL for α-amylase inhibition, 0.028 α-glucosidase, 0.059 ACE—angiotensin-converting enzyme). properties separated using Sephadex G10 three fractions obtained. first third These identified LC–MS/MS technique. following amino acid sequences bean (Phaseolus vulgaris) protein: LPIESKWY, FALVAPVGSEPKA, NSILPIESKPWY, RLTDDTEDSMGRA, KKVELEEEVDDWV, those isolated protein had FPQPQPFQ, QPQQPQQPFPQP, WPQQPQQPFPQPQQ, QSQQPQQPFPQPQQ, QFQPQQPQQPFPQP. indicates that 10 % may be used new special nutritional